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目的观察多巴胺 D_1类受体对血管平滑肌细胞胰岛素样生长因子1(IGF-1)受体的影响。方法以A10细胞为研究对象,免疫印迹观察刺激 D_1类受体对 IGF-1受体表达的影响,并初步探讨 D_1类受体对 IGF-1受体影响的机制。结果刺激 D_1类受体可降低 IGF-1受体的表达,D_1类受体阻断剂可完全阻断 D_1类受体激动剂Fenoldopam 对 IGF-1受体的影响,免疫印迹显示运用蛋白激酶 C(PKC)和促分裂原活化蛋白激酶(MAPK)阻断剂后,IGF-1受体表达恢复,提示 PKC 和 MAPK 途径可能参与了 D_1类受体对血管平滑肌细胞 IGF-1受体的影响过程,在 Wistar-Kyoto(WKY)大鼠和自发性高血压大鼠(SHR)血管平滑肌细胞中 D_1类受体激动剂 Fenoldopam 对IGF-1受体表达具有抑制作用,但该抑制作用在 SHR 细胞明显低于 WKY 细胞。结论 D_1类受体对血管平滑肌细胞 IGF-1受体表达具有抑制作用,该作用可能通过 PKC 和 MAPK 途径发挥一系列生理效应。
Objective To observe the effect of dopamine D_1 receptor on insulin-like growth factor 1 (IGF-1) receptor in vascular smooth muscle cells. Methods A10 cells were used as research objects. Immunoblotting was used to observe the effects of D_1 receptors on the expression of IGF-1 receptor and to explore the possible mechanism of D_1 receptors on IGF-1 receptor. Results The stimulation of D 1 receptors decreased the expression of IGF-1 receptor. The D 1 receptor blockers completely blocked the effect of D 1 -like receptor agonist Fenoldopam on IGF-1 receptor. Western blotting showed that protein kinase C (PKC) and mitogen activated protein kinase (MAPK) blockers, the expression of IGF-1 receptor was restored, suggesting that PKC and MAPK pathway may be involved in the process of IGF-1 receptor of vascular smooth muscle cells , D-type receptor agonist Fenoldopam had inhibitory effect on IGF-1 receptor expression in Wistar-Kyoto (WKY) rats and spontaneously hypertensive rats (SHR) vascular smooth muscle cells, but the inhibitory effect was obvious in SHR cells Lower than WKY cells. Conclusions D_1 receptors have an inhibitory effect on the expression of IGF-1 receptor in vascular smooth muscle cells. This effect may exert a series of physiological effects through PKC and MAPK pathways.