Hydrophobic surface modification of chitosan gels by stearyl for improving the activity of immobiliz

来源 :Chinese Chemical Letters | 被引量 : 0次 | 上传用户:li132zhihua
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
The hydrophobic surface modification of chitosan gels was carried out using the amidating reaction of amido groups on a gel surface with stearic acid activated by 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride(EDC) and N-hydroxysuccinimide (NHS).Lipases from Candida rugosa were adsorbed on the nascent chitosan gels(CS) and stearyl-modified gels(SCS) with different degrees of amidation.The increased surface hydrophobicity of chitosan gels improved the adsorption capacity and activity of the immobilized lipase.SCS with 31.46%amidation showed the maximum activity retention(83.43%).The experimental results suggest that the moderate surface hydrophilicity/hydrophobicity of chitosan gels is necessary for the improvement of the activity of immobilized lipase. The hydrophobic surface modification of chitosan gels was carried out using the amidating reaction of amido groups on a gel surface with stearic acid activated by 1-ethyl-3- (3-dimethylaminopropyl) -carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) . Lipases from Candida rugosa were adsorbed on the nascent chitosan gels (CS) and stearyl-modified gels (SCS) with different degrees of amidation. The increased surface hydrophobicity of chitosan gels improved the adsorption capacity and activity of the immobilized lipase. SCS with 31.46 % amidation showed the maximum activity retention (83.43%). The experimental results suggest that the moderate surface hydrophilicity / hydrophobicity of chitosan gels is necessary for the improvement of the activity of immobilized lipase.
其他文献
公元前597年,一场灭门惨案成就了“赵氏孤儿”的千古传奇。程婴之忠、之烈;公孙杵臼之仁、之义;屠岸贾之奸、之佞;庄姬之屈、之辱;孤儿之刚、之痛,书写了历史上一曲悲壮赞歌
平时,我们照相的时候,为了笑得更开心些,大家一定会情不自禁地说“茄子”。今天,我就给大家说说茄子。茄子是一年生草本植物,叶椭圆形,花紫色,果实球形或长圆形,紫 Usually,
期刊
在毛主席无产阶级革命路线指引下,我省群众性的林业科学试验蓬勃发展。为了适应形势发展的需要,提高试验质量,促进林业生产的发展,我们根据自己的经验,参考有关资料,对进行
目的合成非病毒基因载体材料聚谷氨酸-聚丙烯亚胺,并考查其与DNA的结合能力及稳定性。方法以G2.0聚丙烯亚胺(PPI)为引发剂,通过开环聚合的方法,合成了聚丙烯亚胺-聚谷氨酸苄
High-purity aluminum titanate was synthesized via a water quenching method with waste-residue in the aluminum factory and industrial TiO2 as the main raw materi
句子的语气之所以值得一谈,是因为它在表达感情、体现态度方面具有一种特殊的效果。试看下面两组句子从语气上显示出来的不同作用: The tone of the sentence is worth dis
五年投资466亿建设19项农业和水利工程省委农办副主任陈祖煌表示,今年广东的“三农”工作一方面存在困难和压力,另一方面也蕴含着发展的机遇和潜能。各 Five-year investmen
中学体育教学中会发生学生受伤的情况,这会给学生的生理和心理健康带来一些不良的影响。探讨体育教学中常见运动损伤的原因,以及如何采取合理有效的安全措施,做好各项预防工
2010年的贺岁档,姜文、陈凯歌等五大导演的新片来势汹汹,国内影坛势必呈现出一派史无前例的大片井喷盛况。因此,《让子弹飞》的片方也自嘲自己遭遇了“史上最惨烈贺岁档”。
纵使将实施经济刺激计划后的效果估计得再充分,也只是减缓了经济短期内的下行风险,并不能必然带动经济持续回暖。 Estimation of the effect of implementing the stimulus