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目的:了解缺氧预处理对肢体制动大鼠骨骼肌萎缩的作用。方法:将60只健康、体重150~200g的雌性SD大鼠按体重配对原则随机分为对照组(CON)、大鼠右后肢石膏固定组(IB)、缺氧预处理+大鼠右后肢石膏固定组(HCP)三组,每组20只。HCP组大鼠连续进行1周的缺氧预处理。每天处理的方法为:氧浓度10%,每次处理30分钟,间隔5分钟,连续处理5次。缺氧预处理完成后,将IB及HCP组大鼠右后肢石膏固定3周。实验结束处死三组大鼠,取双后肢比目鱼肌,分别称重。于比目鱼肌中段切取部分肌肉组织,制作石蜡切片,HE染色后用自动图像分析仪测定肌细胞直径及截面积,取部分比目鱼肌匀浆液上清液用于超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量的检测。结果:大鼠右后肢石膏固定3周后,IB组大鼠右后肢比目鱼肌湿重、肌细胞直径及肌纤维截面积明显低于CON组及HCP组。与IB组相比,HCP组比目鱼肌SOD活性明显升高,MDA水平明显降低。结论:缺氧预处理可提高大鼠骨骼肌的抗氧化能力,对肢体制动大鼠骨骼肌萎缩有预防作用。
Objective: To understand the effect of hypoxic preconditioning on skeletal muscle atrophy in limbs braked rats. Methods: Sixty healthy female SD rats weighing 150 ~ 200g were randomly divided into control group (CON), right hind limb plaster fixation group (IB), hypoxic preconditioning + right hind limb gypsum Fixed group (HCP) three groups, each group of 20. HCP rats were continuously subjected to hypoxia preconditioning for 1 week. The daily treatment method is: oxygen concentration of 10%, each treatment for 30 minutes, interval of 5 minutes, continuous treatment 5 times. After hypoxic preconditioning, right hind limb plaster was fixed in IB and HCP rats for 3 weeks. Three rats were sacrificed at the end of the experiment, and the soleus soleus muscles were weighed separately. Part of the muscle tissue was excised from the mid-soleus muscles and paraffin sections were made. After HE staining, the diameter and cross-sectional area of myocytes were measured with an automatic image analyzer. Part of the soleus muscle homogenate supernatant was used for superoxide dismutase (SOD) Malondialdehyde (MDA) content of the test. Results: Three weeks after the right hind limb plaster was fixed, the right hind limb sole muscle wet weight, muscle cell diameter and myofiber cross-sectional area in IB group were significantly lower than those in CON group and HCP group. Compared with IB group, HCP group soleus muscle SOD activity was significantly increased, MDA levels were significantly lower. Conclusion: Hypoxic preconditioning can improve the antioxidant capacity of rat skeletal muscle and prevent the rat atrophy of skeletal muscle.