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目的观察地黄寡糖对2型糖尿病大鼠糖脂代谢的影响及肝脏的保护作用。方法取♂SD大鼠用高脂高糖饲料喂养8周后,腹腔注射链脲佐菌素25 mg·kg?1制备糖尿病大鼠模型,造模成功后随机分组为:糖尿病模型组、地黄寡糖高剂量组、地黄寡糖中剂量组、地黄寡糖低剂量组、二甲双胍组,每组10只;另取8只用普通大鼠饲料喂养8周后,腹腔注射等体积柠檬酸-柠檬酸钠缓冲液,作正常对照组。在第0,2,4,8周末各测定一次空腹血糖(FBG);第8周末测定血清甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、和空腹血清胰岛素(FINS);实验结束处死大鼠,取肝组织检测谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)水平;HE染色后光镜观察肝脏病理变化;透射电镜观察肝脏超微结构的改变。结果与糖尿病模型组比较,地黄寡糖中、高剂量组FBG、TG、TC、LDL-C、FINS水平均明显降低,HDL-C升高(P<0.05);糖尿病大鼠肝组织氧化应激水平增高,地黄寡糖中、高剂量组糖尿病大鼠SOD、GSH-Px活性均显著增加,MDA水平显著下降;HE染色显示,各地黄寡糖治疗组大鼠肝细胞脂肪变性明显减少,肝细胞排列基本规则。透射电镜结果显示,地黄寡糖高剂量组大部分线粒体结构清晰内质网完整。结论地黄寡糖可降低糖尿病大鼠血糖、血脂,减轻肝细胞脂肪变性,从而达到保护肝脏的作用,其作用机制可能与其增强肝组织抗氧化作用,减缓糖尿病对肝组织的损伤有关。
Objective To observe the effect of Rehmannia glutinosa oligosaccharide on glucose and lipid metabolism in type 2 diabetic rats and its protective effect on liver. Methods Male SD rats were fed with high-fat and high-sucrose diet for 8 weeks, and diabetic rats were induced by intraperitoneal injection of streptozotocin (25 mg · kg -1). The rats were randomly divided into diabetic model group, High glucose group, medium dose of Rehmannia glutinosa oligosaccharide, low dose of Rehmannia glutinosa oligosaccharide group and metformin group, and another 8 rats were fed with normal rat diet for 8 weeks. Then, an equal volume of citric acid-citric acid Sodium buffer for normal control group. Fasting blood glucose (FBG) was measured at the end of the 0, 2, 4 and 8 weeks. Serum triglyceride (TG), total cholesterol (TC), low density lipoprotein (LDL- (HDL-C) and fasting serum insulin (FINS). At the end of the experiment, rats were sacrificed and the activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) (MDA). The pathological changes of liver were observed under light microscope after HE staining. The changes of liver ultrastructure were observed under transmission electron microscope. Results Compared with diabetic model group, the levels of FBG, TG, TC, LDL-C and FINS in Rehmannia glutinosa oligosaccharides group were significantly decreased and HDL-C levels were increased (P <0.05). The oxidative stress The levels of SOD, GSH-Px and MDA in diabetic Rehmannia glutinosa oligosaccharides group were significantly increased, while the levels of MDA were significantly decreased. The HE staining showed that the fatty degeneration of hepatocytes in the group treated with Xylose oligosaccharide was significantly reduced, Arrange the basic rules. TEM results showed that most of the high dose group Rehmannia glutinosa oligosaccharides mitochondrial structure clear endoplasmic reticulum integrity. Conclusion Rehmannia glutinosa oligosaccharides can reduce blood glucose, blood lipids, reduce fatty degeneration of liver cells and thus protect the liver, and its mechanism may be related to its role in enhancing the antioxidation of liver tissue and decreasing the damage to liver tissue caused by diabetes.