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目的:观察运脾温阳方及拆方对正常和大黄致脾虚小鼠脾细胞增殖的影响。方法:采用正常和15%大黄水煎液灌胃造成脾虚模型的小鼠,制作脾细胞悬液经培养后,加入ConA或LPS各50μL后,加入运脾温阳方及拆方50μL,培养72h后用MTT检测。结果:运脾温阳方及拆方对正常小鼠和刀豆蛋白诱导脾细胞增殖具有明显的促进作用,对脂多糖诱导的正常小鼠脾细胞增殖未见有明显的促进作用。运脾温阳方及拆方的各剂量组体外对脾虚小鼠、脂多糖和刀豆蛋白诱导的脾细胞增殖都具有明显的促进作用。拆方中各药作用效果均低于全方组。结论:运脾温阳方及拆方具有促进B细胞和T细胞增殖的作用。
OBJECTIVE: To observe the effects of Yunpi Wenyang Recipe and its decoction on proliferation of spleen cells in spleen-deficient mice induced by normal and rhubarb. METHODS: Normal and 15% rhubarb decoction was used to induce spleen deficiency in mice. After the spleen cell suspension was cultured, 50 μL each of ConA or LPS was added, and then 50 μL of spleen-wrapping formula and 50 μL of disassembled formula were added and cultured for 72 h. After the detection with MTT. RESULTS: Yunpi Wenyang Decoction and Decoction had significant effects on the proliferation of normal mice and concanavalin-induced spleen cells, but no significant effect on the proliferation of spleen cells induced by lipopolysaccharide in normal mice. Each dose group of Yunpi Wenyang Fang and Difang has obvious promoting effect on spleen cell proliferation induced by spleen deficiency mice, lipopolysaccharide and Concanavalin in vitro. The effect of each drug in the disassembled prescription was lower than that of the whole group. Conclusion: Yunpi Wenyang Decoction and Decoction can promote the proliferation of B cells and T cells.