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[目的]为进一步提高辣椒花药培养效率。[方法]采用MS培养基,以博辣娇红为试材,研究碳源种类与浓度对花药愈伤组织诱导的影响;以佳玉为试材,按L16(45)正交试验设计,研究植物生长调节剂种类与浓度对辣椒花药培养效率的影响。[结果]愈伤组织和胚状体诱导率各浓度处理的均值麦芽糖虽略高于蔗糖,但未达差异显著水平;麦芽糖或蔗糖作碳源,3%～6%的浓度有利于提高愈伤组织与胚状体的诱导频率,超过6%以后,愈伤组织和胚状体诱导率随糖浓度的增加而极显著下降。生长调节剂对愈伤组织诱导率的影响为2,4-D﹥ZT﹥NAA﹥KT﹥6-BA;对胚状体诱导率的影响为2,4-D﹥NAA﹥ZT﹥KT﹥6-BA;2,4-D、ZT、NAA和KT对愈伤组织与胚状体诱导率均有显著或极显著影响;生长调节剂的使用浓度,2,4-D和ZT以1.0mg/L、NAA和KT对0.5mg/L的效果最好,ZT对提高愈伤组织和胚状体诱导率的效果比KT和6-BA都好;2,4-D1.0mg/L﹢ZT1.0mg/L﹢KT0.5mg/L﹢6-BA0.5mg/L是佳玉花药愈伤组织和胚状体诱导培养适宜的生长调节剂组合。[结论]该研究为辣椒花药培养研究提供了依据。
[Objective] In order to further improve the efficiency of pepper anther culture. [Method] The effects of carbon source and concentration on the induction of anther callus were studied by using MS culture medium with Bola Jiaohong as test material. By using Jade as test material and L16 (45) orthogonal design, Effect of Plant Growth Regulator Species and Concentration on Anther Culture Efficiency of Hot Pepper. [Result] The average maltose concentration of callus and embryoid body at different concentration treatments was slightly higher than that of sucrose, but the difference was not significant. Maltose or sucrose as carbon source, 3% ~ 6% The induction frequency of tissue and embryoid body, when more than 6%, the induction rate of callus and embryoid body significantly decreased with the increase of sugar concentration. The effect of growth regulators on the induction rate of callus was 2,4-D> ZT> NAA> KT> 6-BA. The effect on the embryoid body induction rate was 2,4-D> NAA> ZT> KT> 6 -BA; 2,4-D, ZT, NAA and KT had significant or extremely significant effects on the induction rate of callus and embryoid body; the concentrations of growth regulators, 2,4-D and ZT were 1.0mg / L, NAA and KT on 0.5mg / L the best, ZT on the callus and embryoid body induction rate than KT and 6-BA are better; 2,4-D1.0mg / L + ZT1. 0mg / L + KT0.5mg / L + 6-BA0.5mg / L is a suitable growth regulator combination for callus induction and embryoid induction of Jade Jade. [Conclusion] This study provided the basis for the research of pepper anther culture.