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目的了解广州地区首次参加无偿献血人群HCV的感染状况。方法抗-HCV检测采用酶联免疫吸附实验(使用2种不同厂家的试剂盒),HCV RNA检测采用荧光定量PCR法,ALT检测采用速率法。结果2004—2007年在广州血液中心首次参加无偿献血的559890名献血者中,1617名经双试剂检测抗-HCV阳性,阳性率为0.289%。随机选取435名双试剂检测抗-HCV阳性血液标本进行RNA检测,266名阳性,占61.15%,男女(P<0.01)HCV RNA阳性率有统计学意义(P<0.01)。266名抗-HCV检测阳性献血者中,ALT升高(>40U)的为18名(其中男性17名),不合率为6.77%,男女ALT的不合格比有统计学意义(P<0.01)。结论随着无偿献血的开展,献血人群中HCV的阳性率不断下降;不同性别的个体清除HCV的能力不同,女性高于男性。
Objective To understand the infection status of HCV in Guangzhou for the first time. Methods Anti-HCV test using enzyme-linked immunosorbent assay (using two different manufacturers kit), HCV RNA detection using quantitative PCR, ALT detection rate method. Results Of the 559,890 blood donors who participated in the blood donation for the first time in Guangzhou Blood Center from 2004 to 2007, 1617 were tested positive for anti-HCV by double reagents with a positive rate of 0.289%. A total of 435 anti-HCV positive blood samples were randomly selected for RNA detection. 266 were positive, accounting for 61.15%. The positive rate of HCV RNA in both sexes was statistically significant (P <0.01). Among 266 anti-HCV positive blood donors, there were 18 (including 17 males) with ALT elevation (> 40U) and the rate of non-compliance was 6.77%. There was a statistically significant difference in male and female ALT (P <0.01) . Conclusion With the development of unpaid blood donation, the positive rate of HCV in blood donors declines continuously. Individuals of different genders have different abilities to clear HCV, and women are higher than men.