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一、急性毒性试验 1.予初毒性试验:取体重17～20克健康小自鼠五只以0.7ml/20g体重毒蜂蜜灌服,有毒者一般在24～48小时内死亡。 2.小白鼠半数致死量(LD_(50))测定: 取体重17～20g健康小白鼠20只,分成二组。分别以高剂量0.7ml/20g体重和低剂量0.5ml/20g体重观察72小时。最后按寇氏公式LD_(50)=L0g~(-1)[Xm—i(∑p—0.5)]计算其LD_(50)的结果。小白鼠灌服毒蜂蜜其半数致死量为30.26ml/kg±3.483ml/kg,即有毒蜂蜜半数致死量(LD_(50))一般在26.78至33.74ml/kg范围内。二、溶血试验:取2％家兔红细胞生理
First, the acute toxicity test 1. To early toxicity test: take a body weight of 17 to 20 grams of five small healthy mice fed with 0.7ml/20g body weight of toxic honey, poisonous people generally within 24 to 48 hours of death. 2. Determination of the lethal dose (LD_(50)) of mice: Twenty healthy mice weighing 17 to 20 g were divided into two groups. 72 hours were observed at a high dose of 0.7 ml/20 g body weight and a low dose of 0.5 ml/20 g body weight. Finally, the LD_(50) result is calculated according to the 寇 公式 LD_(50)=L0g~(-1)[Xm−i(∑p−0.5)]. The median lethal dose of mice poisoned with poison honey was 30.26ml/kg±3.483ml/kg, that is, the half lethal dose of toxic honey (LD_(50)) was generally in the range of 26.78 to 33.74ml/kg. Second, hemolysis test: take 2% of rabbit erythrocyte physiology