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以长白忍冬的幼嫩茎段为外植体,MS为基本培养基,添加不同的植物生长调节物质,进行离体快速繁殖技术研究。结果表明:在0.1%HgCl_2溶液中滴加0.1%吐温80处理5min的灭菌效果最好;诱导长白忍冬侧芽分化的适宜培养基为MS+1.0mg·L~(-1) 6-BA+0.05mg·L~(-1) NAA+30g·L~(-1)蔗糖+7g·L~(-1)琼脂或不加任何生长调节物质的MS+30g·L~(-1)蔗糖+7g·L~(-1)琼脂;增殖培养基为MS+1.0mg·L~(-1) 6-BA+0.05mg·L~(-1) NAA+30g·L~(-1)蔗糖+7g·L~(-1)琼脂,30d的增殖系数为8;最佳的生根培养基为1/2MS+15g·L~(-1)蔗糖+7g·L~(-1)琼脂,生根率达60.0%。
Using the young stem segments of Lonicera japonica as explants, MS was the basic medium, and different plant growth regulators were added to study the rapid propagation in vitro. The results showed that 0.1% Tween 80 and 0.1% Tween 80 for 5 min had the best sterilization effect. The suitable medium for inducing lateral bud differentiation of Lonicera japonica Thunb. Was MS + 1.0 mg · L -1 6-BA + 0.05 mg · L -1 NAA + 30 g · L -1 sucrose + 7 g · L -1 agar or MS + 30 g · L -1 sucrose + 7g · L -1 agar and the proliferation medium was MS + 1.0mg · L -1 6-BA + 0.05mg · L -1 NAA + 30g · L -1 sucrose + 7g · L ~ (-1) agar, and the multiplication coefficient was 30 on 30d. The best rooting medium was 1 / 2MS + 15g · L -1 sucrose + 7g · L -1 agar, Up to 60.0%.