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目的:探讨桦褐孔菌乙醇提取物对哮喘小鼠气道炎症和Thl/Th2类细胞因子变化的影响及其可能机制。方法:雄性BALB/c小鼠32只随机分成4组,即正常对照组、哮喘模型组和桦褐孔菌乙醇提取物低、高剂量干预组。分别采用酶联免疫吸附法(ELISA)和蛋白质印迹检测支气管肺泡灌洗液(BALF)中IL-4,IL-5,IL-13,IFN-γ含量以及肺组织中磷酸化p38MAPK的变化,并观察BALF中炎症细胞和肺组织病理学改变。结果:哮喘模型组小鼠BALF中炎症细胞计数,IL-4,IL-5,IL-13水平和IFN-γ水平降低;肺组织中磷酸化p38 MAPK表达水平升高,与正常组比较差异显著(P<0.05)。桦褐孔菌乙醇提取物低、高剂量干预组小鼠BALF中炎症细胞计数,IL-4,IL-5,IL-13水平和肺组织中磷酸化p38MAPK表达水平均明显降低,IFN-γ水平明显上升,与哮喘模型组比较均具有显著差异(P<0.05)。桦褐孔菌乙醇提取物处理能明显减轻哮喘小鼠肺组织病理学改变,桦褐孔菌低、高剂量干预组之间比较无显著差异。结论:p38 MAPK可能参与了支气管哮喘的发病过程。桦褐孔菌对哮喘小鼠具有保护作用,其机制可能与抑制p38 MAPK磷酸化、调节IL-4/IFN-γ平衡失调、减轻炎症细胞浸润有关。
Objective: To investigate the effects of Ethanol extract of Inonotus obliquus on the changes of airway inflammation and Th1 / Th2 cytokines in asthmatic mice and its possible mechanism. Methods: Thirty-two male BALB / c mice were randomly divided into four groups: normal control group, asthma model group and ethanol extract of Inonotus obtiae. The levels of IL-4, IL-5, IL-13 and IFN-γ in bronchoalveolar lavage fluid (BALF) and the changes of phosphorylated p38MAPK in lung tissue were detected by enzyme-linked immunosorbent assay (ELISA) The pathological changes of inflammatory cells and lung tissue in BALF were observed. Results: The number of inflammatory cells, the level of IL-4, IL-5, IL-13 and the level of IFN-γ in BALF were decreased in asthmatic model group. The level of phosphorylated p38 MAPK in lung tissue was increased in asthmatic model group (P <0.05). Inflammatory cells, IL-4, IL-5, IL-13 and phosphorylated p38MAPK expression in BALF of low and high dose intervention groups of Ethanol extract of Inonotus obliquus were significantly decreased, and the level of IFN-γ Significantly increased compared with the asthma model group were significantly different (P <0.05). Ethanol extract of Inonotus obliquus could significantly reduce the pathological changes of lung tissue in asthmatic mice. There was no significant difference between low and high dose groups of Inonotus obliquus. Conclusion: p38 MAPK may be involved in the pathogenesis of bronchial asthma. Inonotus obliquus has a protective effect on asthmatic mice, and its mechanism may be related to inhibition of phosphorylation of p38 MAPK, regulation of imbalance of IL-4 / IFN-γ and alleviation of inflammatory cell infiltration.