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紫孢侧耳、糙皮侧耳和佛罗里达侧耳的原生质体制备与再生的最佳条件为：用１％溶壁酶＋０．５％蜗牛酶的混合酶液酶解培养３～４天的菌丝体，１．５小时后，原生质体产量均可达１０￣（１０）个／Ｌ；三种侧耳的最佳再生培养基分别为ＳＭ、ＭＰ和ＲＰ，再生率分别为０．６１％、０．６７％和０．７６％。随着紫外线对原生质体处理时间的加长，再生率逐渐下降，当处理４ｍｉｎ时，再生率接近零。
The optimum conditions for the preparation and regeneration of protoplasts from Pleurotus citrinopileatus, Pleurotus ostreatus and Florida Pleurotus ostreatus were as follows: the mycelium was cultured for 3 to 4 days by enzymolysis with 1% lywallzyme + 0.5% After 1.5 hours, the protoplast yield reached 10 ~ (10) / L. The best regeneration media of three lateral ear were SM, MP and RP respectively, with the regeneration rates of 0.61% and 0.67 % And 0.76%. With UV light protoplasts processing time lengthened, the regeneration rate decreased gradually, when treated 4min, the regeneration rate close to zero.