论文部分内容阅读
目的了解深圳市福田区感染性腹泻患者中沙门菌的感染现状。方法收集福田区辖内医院2014年9月—2015年11月疑似和确诊为感染性腹泻病人412例的粪便样本,对全部样本进行临床和流行病学因素调查。采用传统分离培养和荧光定量PCR相结合方法进行沙门菌分离鉴定,同时对阳性菌株进行血清学分型。结果 412份粪便样本中共检出50株沙门菌,检出率为12.14%,其中检出最多的型别为肠炎沙门菌,其次为山夫登堡沙门菌、斯坦利沙门菌、鼠伤寒沙门菌以及利齐菲尔德沙门菌。采用传统分离培养和荧光PCR检出的阳性菌分别为40株和37株。阳性样本来自于各个年龄段病人;共检出男性20株,女性30株;阳性样本大便性状以稀便居多(31/50),其次为水样便、粘液便和蛋花便;每日腹泻次数均在2~10次范围内,其中25例阳性样本来自伴有腹痛或呕吐症状的患者。结论近几年深圳地区的沙门菌感染发病率居高不下,且型别多样化,应加强对本地区沙门菌病原谱构成及流行趋势的监测。
Objective To understand the status of Salmonella infection in patients with infectious diarrhea in Futian District, Shenzhen. Methods Stool samples of 412 suspected and confirmed cases of infectious diarrhea in Futian district hospital were collected from September 2014 to November 2015, and clinical and epidemiological investigation of all the samples were performed. Salmonella was isolated and identified by a combination of traditional culture and fluorescence quantitative PCR, and the serotypes of positive strains were also identified. Results A total of 412 salmonella strains were detected in 412 stool samples, with a detection rate of 12.14%. The most common type was Salmonella enteritidis, followed by Salmonella sainfing, Salmonella stanley, Salmonella typhimurium, As well as Salmonella Lichfield. The positive bacteria isolated by traditional separation and fluorescence PCR were 40 and 37, respectively. Positive samples were from patients of all ages. Twenty males and 30 females were detected. The stools of the positive samples were mostly stools (31/50), followed by watery stools, mucus and stools, and daily diarrhea The number of times was in the range of 2 to 10 times, of which 25 cases of positive samples from patients with abdominal pain or vomiting. Conclusion In recent years, the incidence of Salmonella in Shenzhen remains high, and its genotypes are diversified. Therefore, it is necessary to strengthen the monitoring of the composition and epidemic trend of Salmonella in this area.