应用CATS法分离和鉴定猪GFAP基因的研究

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根据比较锚定序列示踪(CATS)法,选择人和小鼠胶质细胞原纤维酸性蛋白(GFAP)基因的同源区域设计引物,用PCR方法从二花脸猪基因组中分离到412bp的基因片段,经与基因资料库中已知功能基因的同源性比较,该片段可鉴定为猪的GFAP基因,利用猪-啮齿类体细胞杂种克隆板将CFAP基因定位于猪12号染色体12p11-(2/3)p13区域。
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