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目的:探讨羟苯磺酸钙对肾小球细胞间黏附分子(ICAM-1)和血管细胞黏附分子(VCAM-1)表达的影响和对糖尿病肾病的保护作用。方法:左肾切除后单次腹腔注射50mg·kg-1链脲佐菌素制备糖尿病大鼠模型,糖尿病大鼠随机分成糖尿病组、羟苯磺酸钙治疗组(100 mg·kg-1·d-1)和正常对照组。用RT-PCR及Western印迹的方法检测12周后ICAM-1、VCAM-1表达的改变。结果:与正常对照组相比,糖尿病组及治疗组ICAM-1、VCAM-1表达增加(P<0．01);而治疗组ICAM-1、VCAM-1 表达显著低于糖尿病组(P<0．01)。结论:羟苯磺酸钙可抑制糖尿病大鼠ICAM-1和VCAM-1的表达,延缓糖尿病肾病的发生、发展。
Objective: To investigate the effect of calcium dobesilate on the expression of ICAM-1 and VCAM-1 and its protective effect on diabetic nephropathy. Methods: Diabetic rats were induced by a single intraperitoneal injection of streptozotocin (50mg · kg-1). Diabetic rats were randomly divided into diabetic group and calcium dobesilate group (100 mg · kg-1 · d -1) and normal control group. The changes of ICAM-1 and VCAM-1 expression after 12 weeks were detected by RT-PCR and Western blotting. Results: Compared with normal control group, the expression of ICAM-1 and VCAM-1 in diabetic group and treatment group increased (P <0.01), while the expression of ICAM-1 and VCAM-1 in diabetic group and treatment group was significantly lower than that in diabetic group (P < 0.01). Conclusion: Calcium dobesilate can inhibit the expression of ICAM-1 and VCAM-1 in diabetic rats and delay the occurrence and development of diabetic nephropathy.