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应用RACE技术,从‘大红’苋菜中克隆到1条MYB基因cDNA全长序列,命名为AmMYB1(登录号为KU557504)。AmMYB1基因开放阅读框为723bp,可编码240个氨基酸。其基因组序列与cDNA比对后显示,AmMYB1基因含有1个内含子。生物信息学分析表明,AmMYB1具有2个连续的MYB结构域,是一个典型的R2R3-MYB;同源分析显示,该基因编码的氨基酸序列与甜菜红素相关BvMYB1的一致性最高,达到54%。亚细胞定位结果显示,AmMYB1蛋白定位于细胞核。实时荧光定量PCR分析表明,AmMYB1基因在‘大红’苋菜叶片红色部位的表达量高于绿色部位;在甜菜红素含量高的叶和茎中表达量明显高于根;在光照条件下表达量高于遮光处理;在红叶品种中的表达量高于绿叶品种。研究结果表明,AmMYB1基因可能是苋菜甜菜红素合成途径中重要的正调控因子。
The full-length cDNA of MYB gene was cloned from Amaranth amaranth using RACE technology and named AmMYB1 (accession number KU557504). The open reading frame of AmMYB1 gene is 723 bp, encoding 240 amino acids. Its genome sequence and cDNA showed that the AmMYB1 gene contained one intron. Bioinformatics analysis showed that AmMYB1 has 2 consecutive MYB domains and is a typical R2R3-MYB. Homology analysis showed that the amino acid sequence of AmMYB1 was most consistent with that of BvMYB1 related to beet red pigment (54%). Subcellular localization results showed that AmMYB1 protein localized in the nucleus. Real-time quantitative PCR analysis showed that the expression level of AmMYB1 gene was higher in the red part of amaranth than that in the green part, higher in the leaves and stems with higher berizin content, higher in the light condition In shading treatment; the expression of red leaf varieties is higher than the green leaf varieties. The results showed that AmMYB1 gene may be an important positive regulator in the betaine synthesis pathway of amaranth.