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临床上应用超分割放射治疗疗效不一,为了了解超分割放射的生物学特点,本实验采用200μm直径HeLaS-3球体为模型,从生长、集落形成率和细胞周期变化(用流式细胞计)等方面比较单次放射,每日一次2 Gy常规放射和两组超分割放射对人体肿瘤细胞的增殖和动力学的影响。 结果:1,在相同总剂量条件下,超分割放射比常规放射和单次放射抑制球体生长作用小。2,球体受放射后由胰酶消化分散成单细胞,其集落形成率以超分割放射组为高。与第一结果相一致,提示在五天疗程中细胞有一定修复和再分布发生。3,细胞周期变化检测表明放射后都有G_2+M期细胞比例增加,伴以G_1和S期细胞比例减少。常规放射后G_2+M期细胞比例较为恒定,而超分割放射后波动大。放射后RNA含量无明显变化。 如采用较大直径内含乏氧细胞的球体为模型,在分割放射期间还可能有再充氧发生,实验结果可能和本实验差异较大。
Clinical application of hyperfractionated radiation therapy has different therapeutic effects. In order to understand the biological characteristics of hyperfractionated radiation, a 200 μm-diameter HeLaS-3 sphere was used as a model for this experiment. The growth, colony formation rate, and cell cycle change were determined by flow cytometry. Comparison of single radiation, etc., once a day, 2 Gy conventional radiation and two groups of hyperfractionated radiation on the proliferation and dynamics of human tumor cells. Results: 1. Under the same total dose condition, hyperfractionated radiation was less effective than conventional radiation and single radiation inhibition spheres. 2. After irradiation, the spheres were dispersed into single cells by trypsin digestion, and the colony formation rate was higher in the hyperfractionated radiation group. Consistent with the first result, it was suggested that there was some repair and redistribution of cells during the five-day course of treatment. 3. Changes in cell cycle detection showed that there was an increase in the proportion of G_2+M phase cells after radiation, with a decrease in the proportion of cells in G_1 and S phases. After conventional radiation, the proportion of G_2+M phase cells was relatively constant, but the fluctuation after hyperfractionation was large. There was no significant change in RNA content after radiation. If a large diameter sphere containing hypoxic cells is used as a model, reoxygenation may occur during segmentation of radiation, and the experimental results may differ significantly from this experiment.