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目的 :观察7-二氟甲氧基-5,4’-二甲氧基金雀异黄素(DFMG)对损伤的人脐静脉内皮细胞(HUVE-12)中环氧化酶2(cyclooxygenase-2,C0X-2)蛋白表达的影响及内皮细胞的保护作用。方法 :TNF-α诱导建立人脐静脉内皮细胞(HUVE-12)损伤模型,不同浓度的DFMG进行干预;流式细胞术检测细胞凋亡、Western Blot检测COX-2蛋白表达、ELISA检测E-选择素、单核趋化蛋白-1(Monocyte chemoattractant protein-1,MCP-1)的释放,蛋白定量分析法测定内皮细胞与单核细胞的黏附率。结果 :DFMG呈浓度依赖性阻断损伤的内皮细胞活性的下降、下调COX-2蛋白的表达、降低内皮细胞与单核细胞的黏附率。结论 :DFMG可能通过下调COX-2蛋白表达水平,从而减少单核细胞/内皮细胞黏附作用,减少炎症渗出,抑制炎症反应,保护血管内皮。
OBJECTIVE: To observe the effect of DFMG on cyclooxygenase-2 (COX) in human umbilical vein endothelial cells (HUVE-12) -2) protein expression and endothelial cell protection. Methods: The model of HUVE-12 injury induced by TNF-α was established and the DFMCs were treated with different concentrations of DFMG. The apoptosis was detected by flow cytometry. The expression of COX-2 protein was detected by Western Blot. The expression of E- The release of monocyte chemoattractant protein-1 (MCP-1) and the adhesion of endothelial cells to monocytes were determined by quantitative protein assay. Results: DFMG decreased the activity of endothelial cells in a concentration-dependent manner, decreased the expression of COX-2 protein and decreased the adhesion rate of endothelial cells to monocytes. CONCLUSION: DFMG may reduce the adhesion of monocytes / endothelial cells, reduce the inflammation exudation, inhibit the inflammatory reaction and protect the vascular endothelium by down-regulating the expression of COX-2 protein.