Channel state information of OFDM-STC system is required for maximum likelihood decoding. A subspace-based semi-blind method was proposed for estimating the channels of OFDM-STC systems. The channels are first estimated blindly up to an ambiguity parameter utilizing the nature structure of STC, irrespective of the underlying signal constellations. Furthermore, a method was proposed to resolve the ambiguity by using a few pilot symbols. The simulation results show the proposed semi-blind estimator can achieve higher spectral efficiency and provide improved estimation performance compared to the non-blind estimator.