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目的:利用正交设计优选颈腰康保健贴处方药材的最佳提取工艺。方法:以出膏率和青藤碱含量为指标,采用HPLC法和L9(34)正交试验设计,对提取药材的溶剂用量、提取次数和提取时间进行正交优选。结果:最佳提取工艺为:10倍量70%乙醇,回流提取2次,每次1小时。方法学考察表明,线性为Y=963554X-18560,R=0.9998,表明青藤碱在0.1044μg~0.3550μg内线性关系良好;精密度试验、稳定性试验、重复性试验、回收率试验均良好。结论:该含测方法和处方药材的提取工艺,提取效果好,操作简单,稳定,可靠,可为产品实际生产提供可靠的参考价值。
OBJECTIVE: To optimize the optimum extraction process of prescription medicine by using orthogonal design. Methods: The rate of ointment and sinomenine content were used as indexes. The orthogonal test was carried out by HPLC and L9 (34) orthogonal design. The solvent dosage, extraction times and extraction time were optimized by orthogonal design. Results: The best extraction process: 10 times the amount of 70% ethanol, reflux extraction 2 times, 1 hour each. The methodological study showed that the linearity was Y = 963554X-18560, R = 0.9998, indicating that sinomenine had a good linear relationship in the range of 0.1044μg ~ 0.3550μg. The precision test, stability test, repeatability test and recovery test were all good. CONCLUSION: The method of extraction and the extraction of the prescription herbs, extraction effect is good, the operation is simple, stable and reliable, which can provide reliable reference value for the actual production of the product.