论文部分内容阅读
探讨了１－硝基蒽醌（１－ＮＯ＿２ＡＱ）和１－氨基蒽醌（１－ＮＨ＿２ＡＱ）同时测定和分别测定的色谱条件，提出了在Ｓｈｉｍ－ｐａｃｋＣＬＳＳ－ＳＩＬ色谱柱上，以正庚烷－１，４二氧六环－乙酸乙酯（６５：３５：５（Ｖ／Ｖ）为流动相，流量为１．５ｍｌ／ｍｉｎ，柱温４０℃，以分光光度检测器于２５４ｎｍ处检测时，４ｍｉｎ内使１－ＮＯ＿２ＡＱ与１－ＮＨ＿２ＡＱ分离；５ｍｉｎ使１－ＮＨ＿２ＡＱ与杂质分离；７ｍｉｎ可使１－ＮＯ＿２ＡＱ与杂质分离的高效液相色谱分析法。其绝对检出下限１－ＮＯ＿２ＡＱ为０．０１２μｇ，１－ＮＨ＿２ＡＱ为０．０２６μｇ，变异系数分别为１．１％和０．９６％，回收率分别为９９．７％和１００．２％。
The simultaneous determination of 1-nitroanthraquinone (1-NO 2 AQ) and 1-aminoanthraquinone (1-NH 2 AQ) and the determination of their respective chromatographic conditions were discussed. On a Shim-pack CLS-SIL column, 1, 4 - dioxane - ethyl acetate (65: 35: 5 (V / V) as mobile phase at a flow rate of 1.5 ml / min and a column temperature of 40 ° C at 254 nm with a spectrophotometer. 1-NO 2 AQ and 1-NH 2 AQ were separated within 4 min, 1-NH 2 AQ was separated from impurities at 5 min, and 1-NO 2 AQ and impurities were separated by high performance liquid chromatography at 7 min.The absolute detection limit of 1-NO 2 AQ was 0.012 μg , 1-NH_2AQ was 0.026μg, the coefficient of variation was 1.1% and 0.96%, the recoveries were 99.7% and 100.2% respectively.