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本文首先用波动试验对标准阳性诱变剂和氨化、非氨化焦糖的诱变性进行验证;在此基础上对Ames平板试验显阴性的胺菊酯、三氯杀虫酯和杀虫脒等的诱变性进行测试,以评价波动试验的灵敏度和这些化学物的遗传毒性.实验证明波动试验测出叠氮钠对TA100的最低诱变剂量为0.015μg/ml,比平板试验低16倍;测出氨化及非氨化焦糖对TA98的诱变剂量分别为5μl/ml及10μ1/ml,比平板试验低5～10倍;胺菊酯在不加S9时对TA97仅在波动试验中显示诱变性;三氯杀虫酯在加S9时对TA98也仅在波动试验中显示诱变性;波动试验还测出了杀虫脒加S9时对TA97有诱变性.
In this paper, firstly, the mutagenicity of standard positive mutagens and ammoniated and non-ammoniated caramel was verified by wave test. On the basis of this, we tested the susceptibility of Ames plate test to tetramethrin, Amidine and other mutagenicity test to evaluate the sensitivity of the volatility test and the genotoxicity of these chemicals.It is proved by experiments that the lowest mutagenic dose of sodium azide to TA100 is 0.015μg / ml, which is 16 lower than the plate test Times; Determination of amidated and non-ammoniated caramel TA98 mutagenesis doses were 5μl / ml and 10μ1 / ml, 5 to 10 times lower than the plate test; tetramethrin in the absence of S9 only TA97 fluctuations The test showed mutagenicity; trichloro-insecticide ester showed only mutagenicity to TA98 in the fluctuation test when added with S9; and the volatility test also showed that mutagenized TA9 with chlordimeform plus S9.