合并全身放射损伤的创伤 (放创复合伤 )是一种重要而有代表性的难愈性创伤 ,其难愈机制尚未完全阐明。成纤维细胞是最为重要的组织修复细胞 ,其辐射敏感性较低。为了明确放创复合伤时合并的放射损伤是否对伤口成纤维细胞有直接损伤作用 ,以及这些损伤作用对创伤愈合的影响 ,实验检测了分离、培养的放创复合伤和单纯创伤大鼠皮肤伤口成纤维细胞的增殖、凋亡及其他反映其参与组织修复能力的指标变化。结果发现 ,在去除全身因素和局部因素 ,特别是创伤局部细胞因子和细胞外基质对成纤维细胞的反馈作用后 ,放创复合伤组伤口成纤维细胞增殖力、贴壁力和粘附力均显著弱于单纯创伤组 ,而成纤维细胞的凋亡率则显著增加。这些结果表明 ,全身放射损伤对伤口成纤维细胞有直接损伤作用 ,使其参与组织修复能力显著受抑 ,这是合并全身放射损伤时创伤难愈的重要原因 Trauma combined with whole body radiation (traumatic injury) is an important and representative traumatic injury, and its refractory mechanism has not yet been fully elucidated. Fibroblasts are the most important tissue repair cells with low radiation sensitivity. In order to clarify whether the combined injury caused by radioactive injury is a direct injury to wound fibroblasts, and the effect of these injuries on wound healing, we tested the effects of wound healing and traumatic wound healing Proliferation, apoptosis of fibroblasts and other indexes that reflect their ability to participate in tissue repair. The results showed that after the removal of systemic and local factors, especially the feedback of wound local cytokines and extracellular matrix on fibroblasts, the wound fibroblasts proliferative, adherent and adhesion Significantly weaker than pure trauma group, while the apoptosis rate of fibroblasts increased significantly. These results indicate that systemic radiation injury has a direct injury on wound fibroblasts and significantly inhibits its ability to participate in tissue repair, which is an important cause of wound healing in combination with systemic radiation injury