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黄瓜黑星菌二菌系（即甘肃菌系和沈阳菌系）分生孢子和菌丝生长的适温为２０～２２℃；在１：５的瓜叶汁液中，１２ｈ后甘肃菌系和沈阳菌系的分生孢子萌发率分别为７３％和６９％。而牛粪浸出液对二菌系分生孢子的萌发有一定的抑制作用，萌发率均低于对照。二菌系对碳源和氮源的利用存在明显的差异，如甘肃菌系在葡萄糖、果糖、蔗糖、木糖和乳糖等培养基上生长量为５．７ｍｍ／ｄ以上，而沈阳菌系仅约３１ｍｍ／ｄ；氮源中以硝酸铵、胱氨酸对甘肃菌系生长的刺激作用明显，生长量分别为６４ｍｍ／ｄ和５８ｍｍ／ｄ，而沈阳菌系仅为３５ｍｍ／ｄ和３４ｍｍ／ｄ。但精氨酸则对二菌系的生长有抑制作用，日生长量低于对照５０％以上。
The suitable temperature for the growth of conidia and hyphae of two strains of Neisseria gonorrhoeae (ie, Gansu strain and Shenyang strain) was 20 ~ 22 ℃. In the melon leaf juice of 1: 5, Gansu strain and Shenyang Conidia germination rate of strains were 73% and 69%. The cow manure leachate germination of the second strain of conidia have a certain inhibitory effect, the germination rate was lower than the control. Two strains of bacteria on the use of carbon and nitrogen sources were significantly different, such as strains of Gansu in glucose, fructose, sucrose, xylose and lactose and other media growth of 5.7mm / d or more, while the Shenyang strain only About 3 1mm / d; ammonium nitrate, cystine on Gansu bacteria growth significantly stimulated growth were 6 4mm / d and 5 8mm / d, while the Shenyang strain was only 3 5mm / d and 3 4mm / d. However, arginine inhibited the growth of the second strain and the daily growth was lower than 50% of the control.