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目的探讨Galectin-3、Fascin-1和β-catenin蛋白在结直肠腺癌(colorectal carcinoma,CRC)组织中的表达及其与临床病理特征的关系。方法应用微波-EliVisionTM(MW-EliVisionTM)免疫组织化学染色技术检测60例CRC、30例结直肠腺瘤和30例正常结直肠黏膜组织中Galectin-3、Fascin-1和β-catenin蛋白的表达,分析其与CRC临床病理特征的关系。结果 Galectin-3、Fascin-1和β-catenin蛋白表达阳性率在CRC组织中分别为68.3%(41/60)、53.3%(32/60)和81.7%(49/60);在腺瘤组织中分别为46.7%(14/30)、30.0%(9/30)和43.3%(13/30);在正常黏膜组织中分别为20.0%(6/30)、3.3%(1/30)和13.3%(4/30),三者在三种组织中表达阳性率间的差异均有统计学意义(P<0.05)。三者的表达与CRC的TNM分期、浸润程度和淋巴结转移有关(P<0.05,P<0.01);Galectin-3和β-catenin蛋白的表达与CRC分化程度有关(P<0.05),而Fascin-1蛋白表达与CRC分化程度无关(P>0.05);三者的表达均与患者年龄、性别及肿瘤大小无关(P>0.05)。Galectin-3蛋白与Fascin-1和β-catenin蛋白表达呈正相关(r=0.728,P<0.01;r=0.696,P<0.01);β-catenin蛋白与Fascin-1蛋白表达呈正相关(r=0.507,P<0.01)。结论 Galectin-3、Fascin-1和β-catenin蛋白在CRC组织中的高表达与其高侵袭能力和淋巴结转移有一定相关性,可作为潜在预测结直肠癌浸润和转移的指标。
Objective To investigate the expression of Galectin-3, Fascin-1 and β-catenin in colorectal carcinoma (CRC) and its relationship with clinicopathological features. Methods The expressions of Galectin-3, Fascin-1 and β-catenin in 60 cases of CRC, 30 cases of colorectal adenoma and 30 cases of normal colorectal mucosa were detected by MW-EliVisionTM immunohistochemical staining. Analysis of its relationship with the clinicopathological features of CRC. Results The positive rates of Galectin-3, Fascin-1 and β-catenin in CRC tissues were 68.3% (41/60), 53.3% (32/60) and 81.7% (49/60) (46/30%), 30.0% (9/30) and 43.3% (13/30) in the normal mucosa tissues, and 20.0% (6/30), 3.3% (1/30) and 13.3% (4/30) respectively. There was a significant difference in the positive expression rates among the three tissues (P <0.05). The expression of Galectin-3 and β-catenin was correlated with the degree of CRC (P <0.05), while the expression of Fascin-3 and β-catenin was correlated with the TNM stage, infiltration and lymph node metastasis 1 protein had no correlation with the degree of CRC (P> 0.05). The expression of all three proteins had no correlation with age, sex and tumor size (P> 0.05). There was a positive correlation between Galectin-3 protein and Fascin-1 and β-catenin protein expression (r = 0.728, P <0.01; r = 0.696, P <0.01) , P <0.01). Conclusions The high expressions of Galectin-3, Fascin-1 and β-catenin in CRC tissues are correlated with their high invasion ability and lymph node metastasis, which may be used as an index to predict the invasion and metastasis of colorectal cancer.