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背景及目的:研究表明,加热对兔VX-2细胞阿霉素化疗有增敏作用,加热可提高细胞内阿霉素的含量。本研究探讨介入性热化疗对正常肝组织及肿瘤肝组织血管渗透性的影响。方法:建立可供实验用的兔VX-2移植性肝癌模型30只,随机分为3组:非灌注组(插管后只注射1%伊文思蓝),常温灌注组(灌注液为25℃生理盐水)。热灌注组(灌注液为60℃生理盐水)。使用标准曲线和分光光度法测量各组组织中伊文思蓝含量(作为血管渗透性的指标)。结果:肿瘤组织与肝组织的EB含量在3组中均有差别(P<0.05);正常灌注组与非灌注组肝组织的EB含量、肿瘤组织的EB含量无明显差别(P>0.05);热灌注组与非灌注组、正常灌注组肝组织的EB含量、肿瘤组织的EB含量有明显差别(P<0.05)。结论:介入性热化疗可以增加肝组织及肿瘤组织的血管渗透性。
BACKGROUND & AIM: Studies have shown that heating can sensitize doxorubicin chemotherapy to VX-2 cells in rabbits and heating can increase intracellular doxorubicin. This study was to investigate the effect of interventional thermochemotherapy on vascular permeability in normal liver tissue and liver tissue. Methods: Thirty rabbits model of VX-2 transplanted liver cancer were established. They were randomly divided into three groups: non-perfusion group (only 1% Evans blue after intubation), normal perfusion group Saline). Heat perfusion group (perfusate 60 ℃ saline). Evan’s blue content (as an indicator of vascular permeability) was measured in each group using standard curve and spectrophotometry. Results: The content of EB in tumor tissue and liver tissue were different in three groups (P <0.05). There was no significant difference in the content of EB and the content of EB in tumor tissue between normal group and non - perfusion group (P> 0.05). The content of EB in liver tissue and the content of EB in tumor tissue in normal perfusion group and non-perfusion group were significantly different (P <0.05). Conclusion: Interventional thermochemotherapy can increase the vascular permeability of liver and tumor tissues.