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目的 探讨AXL基因在正常人、骨髓增生异常综合征 (MDS)及其他血液病患者外周血单个核细胞中的表达情况。方法 采用RT PCR法。结果 ①AXL基因在 10例正常人外周血单个核细胞中不表达 ,正常骨髓单个核细胞中有表达。② 5 0例MDS患者的外周血单个核细胞中有 4 1例 (82 % )AXL基因阳性。FAB亚型与AXL表达关系 :3 5例难治性贫血 (RA)者中 2 7例阳性 ,3例环形铁粒幼细胞性难治性贫血 (RAS)阳性 ;9例难治性贫血伴过多母细胞 (RAEB)者中 8例阳性 ,3例难治性贫血伴过多母细胞伴有转化 (RAEB T)患者均阳性。 6例实验时临床不能确诊为MDS者AXL基因阳性 ,经随访骨髓转为MDS ;3例骨髓疑为MDS或诊为MDS者AXL基因阴性 ,经随访确诊为其他疾病。③ 12例再生障碍性贫血 (AA)、1例缺铁性贫血 (IA)、1例溶血性贫血、2例夜间阵发性血红蛋白尿 (PNH)、3例特发性血小板减少性紫癜 (ITP)患者的AXL均阴性。结论 AXL基因在MDS外周血中有较高表达
Objective To investigate the expression of AXL gene in peripheral blood mononuclear cells of normal people, patients with myelodysplastic syndrome (MDS) and other hematological diseases. Methods RT PCR method. Results ① The AXL gene was not expressed in 10 normal human peripheral blood mononuclear cells and was expressed in normal bone marrow mononuclear cells. ② 41 cases (82%) of the peripheral blood mononuclear cells in 50 MDS patients were positive for AXL gene. The relationship between FAB subtypes and AXL expression: 27 of 35 refractory anemia (RA) patients were positive and 3 of them were positive for RAS; 9 of them had refractory anemia Eight were positive in multiple blast cells (RAEB) and three were refractory anemia with excess of blast cells associated with transformation (RAEB T). 6 cases of clinical trials can not be diagnosed as MDS positive for AXL gene, followed by bone marrow to MDS; 3 cases of bone marrow suspected MDS or diagnosed as MDS AXL gene negative, followed up for the diagnosis of other diseases. ③ 12 cases of aplastic anemia (AA), 1 case of iron deficiency anemia (IA), 1 case of hemolytic anemia, 2 cases of nocturnal paroxysmal hemoglobinuria (PNH), 3 cases of idiopathic thrombocytopenic purpura (ITP ) Patients had negative AXL. Conclusion AXL gene is highly expressed in peripheral blood of MDS