论文部分内容阅读
应用噻唑兰（ＭＴＴ）法测得毛叶茶提取物（ＥＣＰＣ）对人肝癌细胞（ＢＥＬ－７４０２）的半数抑制浓度（ＩＣ＿（５０））为３５１．１μｇ／ｍｌ；台盼蓝拒染法（ＴＢＥ）的ＩＣ＿（５０）为１１５．２μｇ／ｍｌ；用ＭＴＴ法测得ＥＣＰＣ对人红白血病细胞（Ｋ＿（５６２））的ＩＣ＿（５０）＞１０００μｇ／ｍｌ，ＴＢＥ法测得其对Ｋ＿（５６２）细胞的ＩＣ＿（５０）为８１．４μｇ／ｍｌ。ＥＣＰＣ对人胎儿肺纤维细胞（ＨＦＬＦ）和人胎儿肾细胞（ＨＦＫ）显示促生长作用。在药物浓度为６２．５、１２５．０、２５０．０、５００．０和１０００．０μｇ／ｍｌ的培养下，对ＨＦＬＦ细胞的生长促进率分别为—１０．４７、＋１５．４８、＋３１．９１、＋５３．０２和＋８３．３７％：对ＨＦＫ细胞的生长促进率分别为＋１４．１８、＋２６．６１、＋５２．６２、＋８８．１３和＋１０３．７１％。ＦＣＰＣ分别与阿糖胞苷（Ａｒａ—Ｃ）、环磷酰胺（ＣＴＸ）和阿霉素（ＡＤＭ）合用对网织细胞肉瘤（Ｌ＿２）的抑瘤率高于各个单药的抑瘤率。加用ＥＣＰＣ后Ａｒａ—Ｃ可从单用平均抑瘤率３８．２％升高到６４％；ＣＴＸ从平均３１．３％升高到４８．９％；ＡＤＭ从平均１５．２％升高到４５．５％，ｑ?
The half inhibitory concentration (IC_(50)) of human liver cancer cells (BEL-7402) was determined by thiazolyl (MTT) method to be 351.1 μg/ml, and trypan blue dye exclusion method ( The IC 50 of TBE was 115.2 μg/ml. The IC 50 of human erythroleukemia cells (K 562) was measured by MTT method. The IC 50 was more than 1000 μg/ml, and it was measured by TBE method to K 562. The IC_(50) of the cells was 81.4 μg/ml. ECPC shows growth promoting effects on human fetal lung fibroblasts (HFLF) and human fetal kidney cells (HFK). Under the culture conditions of 62.5, 125.0, 250.0, 500.0 and 1000.0 μg/ml, the growth promotion rate of HFLF cells was -10.47, +15.48, +31.91, respectively. , +53.02, and +83.37%: The growth promotion rates for HFK cells were +14.18, +26.61, +52.62, +88.13, and +103.71%, respectively. The inhibitory rate of FCPC with cytarabine (Ara-C), cyclophosphamide (CTX), and adriamycin (ADM) was higher than that of individual drugs. After the addition of ECPC, the Ara-C increased from 38.2% to 64%. The CTX increased from an average of 31.3% to 48.9%. The ADM increased from an average of 15.2%. 45.5%, q?