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自1984年 Izant 和 Weintraub 等首次提出反义技术概念后,反义技术在分子生物学的基础研究进展迅速。近年来,利用反义 RNA 治疗肿瘤在体外实验、动物实验及临床治疗中取得可喜成果。Trojan 等发现反义阻断原癌基因的翻译,Du 等通过反义转染胰岛素生长因子Ⅰ受体(insulin-like growthfactor Ⅰ receptor,IGF-IR)的 cDNA 抑制血管平滑肌细胞生长,Neuenschwandes 等及 Trojan 等分别反义阻断 IGF-ⅠR及胰岛素样生长因子Ⅰ(IGF-Ⅰ)的表达在乳腺癌细胞及神经胶质瘤细胞治疗方面显示了良好的前景。随着肝癌的癌基因、抑癌基因及自分泌生长的深入研究,肝癌的发生具有多病因性多阶段特点,涉及一系列原癌基因的激活和抑癌基因的失活过程。某些自分泌生长因子的过量表达涉及肝癌早期独特的自分泌及旁分泌机制。在肝癌的治疗中,针对肿瘤发生起重要
Since 1984, when Izant and Weintraub proposed the concept of antisense technology for the first time, antisense technology has made rapid progress in the basic research of molecular biology. In recent years, antisense RNA therapy has achieved promising results in in vitro experiments, animal experiments and clinical treatments. Trojan et al. found translation of antisense-blocking proto-oncogenes, Du et al. inhibited the growth of vascular smooth muscle cells by antisense transfection of insulin-like growth factor I receptor (IGF-IR) cDNA, Neuenschwandes et al. and Trojan. Such antisense blocking of IGF-IR and insulin-like growth factor I (IGF-I) expression has shown good prospects in the treatment of breast cancer cells and glioma cells. With the in-depth study of cancer genes, tumor suppressor genes, and autocrine growth of liver cancer, the occurrence of liver cancer has multi-etiological and multi-stage characteristics, involving the activation of a series of proto-oncogenes and the inactivation of tumor suppressor genes. Overexpression of certain autocrine growth factors involves the unique autocrine and paracrine mechanisms of early stage liver cancer. In the treatment of liver cancer, it is important for tumorigenesis