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目的测定溶菌酶对微小小单胞菌及其生物膜的抑菌作用,并测出最小抑菌浓度(MIC)、最小杀菌浓度(MBC)和抑菌率。方法采用对倍稀释的方法,测定溶菌酶对微小小单胞菌的MIC、MBC;在96孔板中体外建立微小小单胞菌生物膜模型,采用MTT法检测溶菌酶对微小小单胞菌生物膜的影响;在六孔板中建立生物膜模型,使用激光共聚焦显微镜(CLSM)观察不同浓度溶菌酶对微小小单胞菌生物膜作用后的变化。结果溶菌酶对微小小单胞菌的MIC为0.0195 mg/mL,MBC为0.3125 mg/mL;CLSM观察结果显示,溶菌酶对微小小单胞菌生物膜的抑制作用随着浓度的增加而增强。结论溶菌酶对微小小单胞菌及其生物膜的生长和活性均具有抑制作用。
Objective To determine the bacteriostasis of lysozyme against Micromonas and its biofilms, and to determine the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) and inhibitory rate. Methods To determine the MIC and MBC of lysozyme against Microcystis microsporum by fold dilution method, the model of C. micromonads biofilm was established in vitro in 96-well plate, and the inhibitory effect of lysozyme on Microcystis aeruginosa Biofilm. The biofilm model was established in a six-well plate, and the changes of the biofilm activities of lysozyme at different concentrations were observed by laser confocal microscopy (CLSM). Results MIC of lysozyme to Micrococcus lomonosus was 0.0195 mg / mL and MBC was 0.3125 mg / mL. The results of CLSM showed that the inhibitory effect of lysozyme on the microbiofilm was enhanced with the increase of concentration. Conclusion Lysozyme has an inhibitory effect on the growth and activity of microorganisms and their biofilms.