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目的:探讨复方隔山消颗粒对胃肠运动的影响及其机制。方法:将实验动物随机分为正常对照组、模型对照组、西沙必利(小鼠0.003 g.kg-1,大鼠0.001 5 g.kg-1)组和高、中、低隔山消颗粒(小鼠:10,5,2.5 g.kg-1,大鼠:5,2.5,1.25 g.kg-1)组。按20 mL.kg-1剂量给药6 d后,用硫酸阿托品抑制小鼠小肠推进运动,测定各组小鼠的小肠推进率;以夹尾刺激法制造大鼠功能性消化不良(FD)模型,按10 mL.kg-1剂量给药7 d,测定各组大鼠胃黏膜一氧化氮(NO)含量和血中胃肠激素水平。结果:西沙必利组和高、中、低隔山消颗粒组小鼠小肠推进率均显著高于模型对照组,西沙必利组和高、中、低隔山消颗粒组大鼠胃黏膜NO含量均明显低于FD模型对照组,西沙必利组和高、中隔山消颗粒组大鼠血清胃泌素(GAS)和血浆胃动素(MTL)含量均明显高于FD模型对照组(P<0.05或P<0.01)。结论:复方隔山消颗粒能促进胃肠运动,其机制可能与其降低胃黏膜NO含量,提高血清GAS和血浆MTL水平有关。
Objective: To investigate the effect of Fufang Xiaoshan Xiaoyu Granule on gastrointestinal motility and its mechanism. Methods: Experimental animals were randomly divided into normal control group, model control group, cisapride (mouse 0.003 g.kg-1, rat 0.001 5 g.kg-1) group and high, middle and low Geosan particles Mice: 10, 5, 2.5 g.kg-1, rats: 5, 2.5, 1.25 g.kg-1). After a dose of 20 mL.kg-1 was administrated for 6 days, atropine sulfate was used to inhibit the small intestine propulsion, and the rate of small intestine was determined in each group. Functional dyspepsia (FD) model was made by tail-tailed stimulation The rats were administered with 10 mL.kg-1 for 7 d, and the content of nitric oxide (NO) and the level of gut hormone in the blood were measured. Results: The intestinal propulsion rate of cisapride group and high, medium and low Geosanxiao granule mice were significantly higher than that of the model control group. The content of NO in cisapride group and high, middle and low Geosanxiao granule group rats were The levels of serum gastrin (GAS) and plasma motilin (MTL) were significantly lower in the FD model control group, cisapride group and high and Zhongsheshanxiao granule group than in the FD model control group (P <0.05 Or P <0.01). CONCLUSION: Compound Wushanxiao Granules can promote gastrointestinal motility, the mechanism may be related to its reduction of gastric mucosal NO content, increase of serum GAS and plasma MTL levels.