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目的:观察草豆蔻挥发油对小鼠急性毒性反应,评价其安全性大小。方法:给小鼠灌服和腹腔注射不同剂量浓度的草豆蔻挥发油,观察小鼠的活动和毒性反应,记录小鼠的死亡数,并用Bliss法计算半数致死量(LD_(50))。结果:草豆蔻挥发油对小鼠经口服及腹腔注射的急性毒性症状主要有:行动迟缓、异步态、心率加快、呼吸急促、连续性抽搐。小鼠口服给药的LD_(50)为237.8 g·kg~(-1)·d~(-1)95%可信限为277.9～203.4 g·kg~(-1)·d~(-1),腹腔给药的LD_(50)为157.9 g·kg~(-1)·d~(-1),95%可信限为176.9～140.9 g·kg~(-1)·d~(-1)。结论:草豆蔻挥发油毒性较低,为进一步开发应用提供依据。
Objective: To observe the acute toxicity of the essential oil of cardamon in mice and to evaluate its safety. Methods: Mice were fed with intraperitoneal injection of different concentrations of cardamom oil, mice activity and toxicity were observed, the number of mice were recorded and the half-lethal dose (LD 50) was calculated by Bliss method. Results: The main toxic symptoms of Ophiopogon volatile oil were oral and intraperitoneal injection of mice: slow, asynchronous, rapid heart rate, shortness of breath, continuous convulsions. The LD_ (50) of oral administration in mice was 237.8 g · kg -1 · d -1 95% confidence interval was 277.9 ~ 203.4 g · kg -1 · d -1 ), The LD 50 of intraperitoneal administration was 157.9 g · kg -1 · d -1, the 95% confidence limit was 176.9-140.9 g · kg -1 · d ~ (-1) 1). Conclusion: The volatile oil of cardamom is relatively low, providing the basis for further development and application.