论文部分内容阅读
目的研究miR-101与其靶基因环氧合酶-2(Cyclooxygenase,COX-2)在维吾尔族妇女慢性宫颈炎、宫颈上皮内瘤样病变Ⅰ、Ⅱ、Ⅲ(CINⅠ、Ⅱ、Ⅲ)及子宫颈癌组织中的表达,以探讨miR-101及其与COX-2的关系。方法应用锁定核苷酸原位杂交技术(LNA-ISH)检测维吾尔族妇女慢性宫颈炎、CINⅠ～Ⅲ和子宫颈癌组织中miR-101的表达情况;应用免疫组化Envision两步法检测COX-2在维吾尔族妇女慢性宫颈炎,宫颈上皮内瘤样病变Ⅰ、Ⅱ、Ⅲ及子宫颈癌组织中的表达情况,并进行相关性分析。结果 miR-101在慢性宫颈炎、CINⅠ～Ⅲ及子宫颈癌中的表达率分别为80%、72%、48%、24%和8%,差异具有统计学意义(χ2=37.828,P=0.000)。慢性宫颈炎组织中未见COX-2阳性表达;CINⅠ、Ⅱ、Ⅲ以及子宫颈癌组织中COX-2阳性表达率分别为12%、36%、60%、84%,差异具有统计学意义(χ2=28.846,P<0.05)。miR-101与其靶基因COX-2呈负相关性(R=-0.997,P=0.000)。结论 miR-101与其靶基因COX-2可能与子宫颈癌的发病机制有关。
Objective To investigate the relationship between miR-101 and its target gene cyclooxygenase-2 (COX-2) in cervical intraepithelial neoplasia (CINⅠ, Ⅱ, Ⅲ) and cervical intraepithelial neoplasia in Uigur women Cancer tissue to explore the relationship between miR-101 and COX-2. Methods The expression of miR-101 in chronic cervicitis, CINⅠ-Ⅲ and cervical cancer tissues was detected by LNA-ISH. In Uygur women with chronic cervicitis, cervical intraepithelial neoplasia lesions Ⅰ, Ⅱ, Ⅲ and cervical cancer, and the correlation analysis. Results The expression rates of miR-101 in chronic cervicitis, CINⅠ-Ⅲ and cervical cancer were 80%, 72%, 48%, 24% and 8% respectively, with statistical significance (χ2 = 37.828, P = 0.000 ). There was no COX-2 positive expression in chronic cervicitis tissues; the positive expression rates of COX-2 in CINⅠ, Ⅱ, Ⅲ and cervical cancer tissues were 12%, 36%, 60% and 84% respectively χ2 = 28.846, P <0.05). There was a negative correlation between miR-101 and its target gene COX-2 (R = -0.997, P = 0.000). Conclusion miR-101 and its target gene COX-2 may be related to the pathogenesis of cervical cancer.