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目的:研究经典古方定志小丸(由石菖蒲、远志、茯苓、人参按2∶2∶3∶3比例组成)对东莨菪碱所致小鼠学习记忆障碍的影响及可能的作用机制。方法:将小鼠随机分为正常组、模型组、阳性对照组(石衫碱甲0.05 mg·kg-1)、定志小丸高、中、低剂量组(700,350,175 mg·kg-1),连续灌胃7 d后,腹腔注射东莨菪碱(1.5 mg·kg-1)制备小鼠学习记忆障碍模型,通过Morris水迷宫试验评价各组小鼠的学习记忆功能,并测定各组小鼠全脑中谷氨酸(Glu)、γ-氨基丁酸(GABA)、5-羟色胺(5-HT)、多巴胺(DA)、乙酰胆碱(Ach)含量及乙酰胆碱酯酶(AchE)活性。结果:行为学测试结果表明定志小丸能明显降低模型小鼠在Morris水迷宫定位航行试验中的平均潜伏期,增加空间探索试验中的穿越平台次数、目的象限游泳路程及时间的百分比;增加小鼠脑中Glu,5-HT,DA和Ach含量,降低GABA含量和AchE活性。结论:定志小丸可明显改善东莨菪碱所致小鼠学习记忆障碍,增强小鼠学习记忆的能力,其作用机制可能与调节Glu/GABA系统以及提高脑中Ach和单胺递质含量有关。
OBJECTIVE: To study the effect of classical ancient prescription Dingzhi Pellets (composed of Acorus calamus, Polygalaceae, Poria cocos, Ginseng at a ratio of 2: 2: 3: 3) on learning and memory impairment induced by scopolamine and its possible mechanism. Methods: The mice were randomly divided into normal group, model group, positive control group (stryedine 0.05 mg · kg -1), Dingzhi Pellet high, medium and low dose groups (700, 350 and 175 mg · kg -1) After 7 days of intragastric administration, scopolamine (1.5 mg · kg-1) was injected intraperitoneally to establish a model of learning and memory impairment in mice. Morris water maze test was used to evaluate the learning and memory function of mice in each group. Glutamic acid Glu, GABA, 5-HT, DA, Ach and AchE activity were measured. Results: The results of behavioral tests showed that Dingzhi Xiaoyan significantly reduced the average latency of model mice in the Morris water maze positioning navigation test, increased the number of crossing platforms in the space exploration test, the target quadrant swimming distance and the percentage of time; increased mice Brain Glu, 5-HT, DA and Ach content, reduce GABA content and AchE activity. Conclusion: Dingzhi Xiaoyan can significantly improve learning and memory impairment of mice induced by scopolamine and enhance the ability of learning and memory in mice. The mechanism may be related to the regulation of Glu / GABA system and the increase of Ach and monoamine transmitters in the brain.