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为进一步提高工程菌的下游纯化效率，本文利用高效表达质粒载体将多拷贝脑钠素基因转人宿主菌E．xoliM5219，摸索最佳发酵条件，热诱导获较高表达（30％）:通过超声裂解收获目的蛋白包涵体，采用制备性电泳成功获得纯度大于95％的5BNP融合蛋白；经BNP／SKATOLE裂解成单体后测得有舒张血管活性。该纯化路线为后续工作提供了线索。
In order to further improve the efficiency of downstream purification of engineered bacteria, multi-copy BNP gene was transferred into host E. coli by using high expression plasmid vector. xoliM5219 to explore the best fermentation conditions, the higher expression of heat-induced (30%): The target protein inclusion bodies were harvested by sonication, and the purity of more than 95% was obtained by preparative electrophoresis. The BNP / SKATOLE was cleaved into single Diastolic measured after vasomotor activity. This purification route provides clues to follow-up work.