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目的观察亚最低杀菌浓度(Sub-minimum bactericidal concentration,Sub-MBC)阿莫西林对金黄色葡萄球菌标准菌株的耐药性影响。方法以金黄色葡萄球菌标准菌株(ATCC 29213)为研究对象,阿莫西林为抗菌药物,以微量稀释法定量检测不同诱导天数MBC值,采用1/2MBC浓度对金黄色葡萄球菌进行30 d多步体外诱导实验,观察金黄色葡萄球菌标准菌株MBC变化,以全自动微生物药敏鉴定仪对最终诱导后菌株进行阿莫西林耐药性分析。结果金黄色葡萄球菌标准菌株MBC值阿莫西林体外诱导7 d即显著升高,诱导30 d升高至原始菌株MBC的32倍;经全自动药敏分析仪鉴定诱导出的耐药菌株对阿莫西林耐药。结论亚最低杀菌浓度阿莫西林可体外诱导金黄色葡萄球菌标准菌株出现耐药,且耐药性随亚最低杀菌浓度诱导时间增加而升高,提示合理选择抗菌药物使用剂量可作为预防细菌耐药重要措施。
Objective To observe the effect of sub-minimum bactericidal concentration (Sub-minimum bactericidal concentration, amoxicillin) on the drug resistance of Staphylococcus aureus standard strains. Methods The standard strain of Staphylococcus aureus (ATCC 29213) was used as the research object. Amoxicillin was used as an antibacterial agent. The MBC value of different induction days was quantitatively determined by microdilution method. Staphylococcus aureus was stained with 1/2 MBC for 30 days In vitro induction experiments were performed to observe the changes of MBC of Staphylococcus aureus standard strains. The antibiotic susceptibility of the finally induced strains was analyzed with an automatic microbial susceptibility detector. Results The methicillin-producing Staphylococcus aureus (MBC) value of amoxicillin was significantly increased in vitro for 7 days and increased to 32 times of the original strain in 30 days after induced by Staphylococcus aureus. The induced drug- Mosicilin resistant. Conclusions Amoxicillin can induce the resistance of Staphylococcus aureus standard strains in vitro and the drug resistance increases with the induction time of sub-minimum bactericidal concentration, suggesting that the rational choice of antimicrobial dosage can be used as a preventive measure against bacterial resistance Important measure