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研究新型雌激素受体ERα36在人肝细胞和肝癌细胞系中的差异表达具有重要的意义.以张氏肝(Chang Liv-er)、Caveolin-1基因沉默细胞株CAV7、人肝癌细胞系SMMC-7721和HepG2为实验材料,通过免疫印迹杂交法观察了不同细胞内ERα36蛋白的表达情况,并与典型雌激素受体亚型ERα66蛋白的表达进行了比较.结果发现:(1)几种细胞中均有ERα36蛋白的表达;(2)与张氏肝细胞相比,Caveolin-1基因沉默时ERα36表达明显增加(P<0.01);(3)ERα36在SMMC-7721中表达水平较低,而在HepG2细胞中表达水平较高,与ERα66和Caveolin-1的表达水平相关.提示新型雌激素受体ERα36可能参与Caveolin-1介导的雌激素信号转导,而在肝细胞的恶性增殖过程中发挥一定的作用.
To investigate the differential expression of ERα36, a novel estrogen receptor, in human hepatocarcinoma and hepatocellular carcinoma cell lines.It is of great significance to study the effect of Chang Liv-er, Caveolin-1 gene silencing cell line CAV7, human hepatoma cell line SMMC- 7721 and HepG2 were used as experimental materials and the expression of ERα36 protein in different cells was observed by Western blot hybridization and compared with the expression of typical estrogen receptor ERα66 protein.The results were as follows: (1) (2) The expression of ERα36 was significantly increased in Caveolin-1 gene silencing compared with Chang’s hepatocytes (P <0.01); (3) The expression level of ERα36 in SMMC-7721 was lower than The expression of ERα36 and Caveolin-1 were higher in HepG2 cells, suggesting that ERα36 may be involved in Caveolin-1-mediated estrogen signaling and play a role in the malignant proliferation of hepatocytes A certain role.