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In order to improve the saccharification rate by acid hydrolysis in the technique for production of ethanol from biomass,in this study,Saccharum spontaneum L. was used as the experimental material to investigate the rules and technological conditions for two-step acid-hydrolysis saccharification by single factor experiment and orthogonal experiment. According to the results,concentrated sulfuric acid hydrolysis was the first step,with the liquid-solid ratio of 42∶ 1,sulfuric acid concentration of 70%,hydrolysis time of 20 min,and hydrolysis temperature of 55 ℃; dilute sulphuric acid hydrolysis was the second step,with the liquidsolid ratio of 115∶ 1,sulfuric acid concentration of 5. 5%,hydrolysis time of 155 min,and hydrolysis temperature of 100 ℃. In two-step acid hydrolysis process,the yield of reducing sugar was 48. 78%. The results indicated that two-step acid-hydrolysis saccharification required mild conditions,simple operation and low cost,and led to high reducing sugar yield,exhibiting a broad application prospect.
In order to improve the saccharification rate by acid hydrolysis in the technique for production of ethanol from biomass, in this study, Saccharum spontaneum L. was used as the experimental material to investigate the rules and technological conditions for two-step acid-hydrolysis saccharification by single factor experiment and orthogonal experiment. According to the results, concentrated sulfuric acid hydrolysis was first step, with the liquid-solid ratio of 42: 1, sulfuric acid concentration of 70%, hydrolysis time of 20 min, and hydrolysis temperature of 55 ° C .; dilute sulfuric acid hydrolysis was the second step, with the liquid-solid ratio of 115: 1, 5% sulfuric acid concentration, hydrolysis time of 155 min, and hydrolysis temperature of 100 ° C. In two-step acid hydrolysis process, the yield of reducing sugar was 48. 78%. The results indicated that two-step acid-hydrolysis saccharification required mild conditions, simple operation and low cost, and led to high reducing sugar y ield, exhibiting a broad application prospect.