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目的 利用银杏悬浮细胞对酯蟾毒配基进行结构修饰。方法 利用只含生长素 2 ,4 D的MS培养基诱导银杏嫩叶 ,使细胞脱分化形成愈伤组织 ,然后将愈伤组织转移至含一定浓度 6 BA ,NAA ,和 2 ,4 D的液体MS培养基中以形成悬浮细胞。把酯蟾毒配基加入生长状态良好的悬浮细胞中转化四天。提取出溶解于液体相的转化产物 ,采用硅胶吸附柱层析法 ,以石油醚和丙酮为展开体系进行梯度洗脱 ,然后对转化产物进行分离纯化。结果 经过四天转化 ,得到一个转化产物 ,转化率达 4 0 % ,通过对转化产物的质谱 ,核磁共振氢谱和碳谱等波谱数据进行分析 ,并与有关文献进行对比 ,证明转化产物为 3 表 酯蟾毒配基。结论以银杏悬浮细胞作为一种生物酶体系 ,可以把来源于动物的蟾蜍甾烯类化合物酯蟾毒配基转化成 3 表 酯蟾毒配基。
Objective To modify the structure of ester glycosides by using Ginkgo biloba suspension cells. Methods Ginseng leaves were induced by MS medium containing only auxin 2 and 4 D. The cells were dedifferentiated to form callus. The calli were then transferred to liquids containing certain concentrations of 6 BA, NAA, and 2,4 D. MS medium to form suspension cells. The ester scorpion venom was added to the suspension cells in a good growth state for four days. The conversion product dissolved in the liquid phase was extracted and subjected to silica gel adsorption column chromatography using petroleum ether and acetone as the eluent system to perform gradient elution, and then the conversion product was isolated and purified. Results After four days of conversion, a conversion product was obtained with a conversion rate of 40%. The spectrum of the conversion product was analyzed by mass spectrometry, nuclear magnetic resonance spectroscopy and carbon spectroscopy, and compared with relevant literature to prove that the conversion product was 3 Table esters toxic ingredients. Conclusion Ginkgo biloba suspension cells can be used as a bio-enzyme system to convert the terpenoids from terpenoids derived from animals into 3-epitoxin ligands.