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鲍曼不动杆菌已成为重要的院内感染病菌。我们测定了鲍曼不动杆菌ATCC 19606的药物最小抑制浓度(M IC),结果显示该菌株对多种抗菌药物都有很高的耐性,如链霉素、诺氟沙星、氯霉素、红霉素、四环素、氨苄西林及一些其他的抗菌染剂。利用大肠埃希菌超敏菌株KAM 32作为宿主,从鲍曼不动杆菌ATCC 19606染色体DNA中克隆耐药基因,共获得9个使宿主细胞产生耐药性的杂合质粒,其中1个为单一耐药,其余全部为多重耐药。根据药物特异性分析可知,具有不同耐药图谱的杂合质粒携带不同类型的耐药基因。由此揭示鲍曼不动杆菌ATCC 19606的多重耐药有多种机制参与。
Acinetobacter baumannii has become an important nosocomial infection bacteria. We determined the minimum inhibitory concentration (MIC) of Acinetobacter baumannii ATCC 19606 and found that the strain is highly resistant to various antimicrobial agents such as streptomycin, norfloxacin, chloramphenicol, Erythromycin, tetracycline, ampicillin and some other antibacterial dyes. Using Escherichia coli hypersensitive strain KAM 32 as the host, the drug resistance genes were cloned from Acinetobacter baumannii ATCC 19606 chromosomal DNA to obtain 9 hybrid plasmids that made the host cells resistant, of which 1 was a single Resistance, the rest are all multi-drug resistance. According to the analysis of drug specificity, hybrid plasmids with different drug resistance maps carry different types of drug resistance genes. Thus, there are many mechanisms involved in the multi-drug resistance of Acinetobacter baumanni ATCC 19606.