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目的利用荧光标记抗人黏病毒蛋白A(MxA)单克隆抗体和流式细胞术早期特异性检测白血病患儿伴发的病毒感染。方法荧光标记抗MxA单克隆抗体,检测其特异性;采集临床发热和不发热的白血病患儿的外周血,分离外周血单个核细胞进行胞内染色,并用流式细胞术检测结果。结果荧光染料直接标记MxA单克隆抗体具有较高的特异性;白血病患儿外周血单核细胞MxA表达水平与其临床病毒感染直接相关,并且检出MxA高表达较临床确诊病毒感染早7d;没有发热和临床确诊为细菌感染的病例均没有检测到MxA的表达。结论荧光标记抗MxA单克隆抗体应用于流式细胞术可以早期特异性地检测白血病患儿伴发的病毒感染,并且此方法简便,易操作,具有临床应用前景。
Objective To detect the virus infection associated with leukemia in early stage by fluorescently labeled MxA monoclonal antibody and flow cytometry. Methods Fluorescently labeled anti-MxA monoclonal antibody was used to detect its specificity. Peripheral blood from children with clinically and non-febrile leukemia was collected. Peripheral blood mononuclear cells were isolated for intracellular staining and the results were analyzed by flow cytometry. Results MxA monoclonal antibody was directly labeled with fluorescent dye. MxA expression in peripheral blood mononuclear cells was directly correlated with clinical virus infection, and MxA expression was detected as early as 7 days after infection with clinically confirmed virus. There was no fever And MxA expression was not detected in any of the clinically diagnosed bacterial infections. Conclusion The application of fluorescent labeled anti-MxA monoclonal antibody in flow cytometry can early and specifically detect the viral infection associated with leukemia in children. Moreover, this method is simple and easy to operate and has a potential clinical application.