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目的 :观察双黄连口服液对人类体外不同靶器官细胞的毒性及特点。方法 :应用人类多器官细胞共培养模型,观察双黄连口服液分别作用24、48、72 h后,对WI-38、HepG2、HEK293、SH-SY5Y和HCF细胞增殖的影响。结果 :双黄连口服液处理24、48、72 h后,各种细胞的增殖率有随着剂量的降低而升高的趋势、且有较好的剂量-效应关系。该药除在72 h后的1/32浓度对HEK293细胞无影响外,在所有时点的各浓度均抑制该细胞的增殖。处理24 h后,1/4~1(原液)浓度的双黄连口服液抑制WI-38细胞,1/8~1(原液)浓度的该药抑制HepG2、SHSY5Y和HCF细胞增殖;48 h后,1/16~1(原液)浓度抑制WI-38、HepG2、SH-SY5Y和HCF细胞;72 h后,1/2~1(原液)浓度的双黄连口服液抑制WI-38细胞,所有浓度的该药均抑制HepG2、SH-SY5Y和HCF细胞。结论 :双黄连口服液对WI-38、HepG2、HEK293、SH-SY5Y和HCF细胞可能均有毒作用,其可能的毒性大小顺序为肾细胞>肝细胞>神经、心肌细胞>肺细胞。
Objective: To observe the toxicity and characteristics of Shuanghuanglian Oral Liquid on human target organ cells in vitro. Methods: The human multi-organ cell co-culture model was used to observe the effects of Shuanghuanglian oral solution on the proliferation of WI-38, HepG2, HEK293, SH-SY5Y and HCF cells after 24, 48 and 72 hours respectively. Results: Shuanghuanglian oral solution 24,48,72 h after treatment, the proliferation rate of various cells with the dose decreased and the trend of increasing, and there is a good dose-response relationship. In addition to its effect on HEK293 cells at a 1/32 concentration after 72 h, the drug inhibits the proliferation of the cells at all concentrations at all times. After 24 h of treatment, Shuanghuanglian oral solution of 1 / 4-1 (liquid concentrate) inhibited the proliferation of WI-38 cells and 1 / 8-1 (stock solution) of HepG2, SHSY5Y and HCF cells. After 48 h, WI-38, HepG2, SH-SY5Y and HCF cells were inhibited by 1 / 16-1 (original solution) concentration, while WI-38 cells were inhibited by Shuanghuanglian 1/2/1 (original solution) This drug inhibits HepG2, SH-SY5Y and HCF cells. Conclusion: Shuanghuanglian Oral Liquid may have toxic effects on WI-38, HepG2, HEK293, SH-SY5Y and HCF cells. The possible toxicity order is kidney cell> hepatocyte> nerve and cardiomyocyte> lung cell.