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经能量为 75Ke V,剂量为 10 14 ions/ cm2的氮、碳离子处理的甜菊种子及未处理的甜菊种子 ,栽培成苗后取叶片 ,提取糖苷后在相同的条件下进行毛细管电泳定量检测 ,研究氮、碳离子处理后主要糖苷成分 Stevioside和 Rebaudioside含量的变化 .结果表明 ,C+ 处理组 Stevioside和 Rebaudioside含量明显地高于对照组 ,N+ 处理组 Stevioside含量也明显地高于对照组 ,而 C+ 处理效果强于 N+ .实验结果还表明 ,用毛细管电泳法研究低能离子处理后糖苷变化的尝试是完全可行的 ,从而建立一种快速、简便而准确的用毛细管电泳筛选甜菊糖苷突变株的新方法 .
Nitrogen, carbon-treated stevia seeds and untreated stevia seeds with an energy of 75 KeV and a dose of 10 14 ions / cm 2 were harvested and harvested for leaf seeding. The glycosides were extracted and quantified by capillary electrophoresis under the same conditions. The results showed that the contents of Stevioside and Rebaudioside in C + treatment group were significantly higher than those in control group, and the content of Stevioside in N + treatment group was also significantly higher than that in control group, while C + treatment And the effect is stronger than that of N + .Experimental results also show that it is feasible to use capillary electrophoresis to study the changes of glycosides after low-energy ion treatment, so as to establish a rapid, simple and accurate method for screening stevioside mutants by capillary electrophoresis.