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已经证明,HBV和控制干扰素(IFN)诱导的抗病毒系统的序列之间具有核苷酸的同源性。作者在本文中研究了不同拷贝数量的HBV基因组在转染细胞中的存在是否降低转染细胞对IFN的敏感性。作者构建了不含HBV基因组和含有2个、12个拷贝的HBV亚型ayw基因组的质粒,并用这些质粒转染对IFN敏感的人羊膜细胞系、FL5-1和HeLa细胞。通过测定转染的FL5-1细胞在IFN诱导前后对Sindbis病毒致病性的抑制作用,计算出抑制率,推测抗病毒反应的高低,还用放射免疫法测定了不同质粒染的HeLa细胞中的β_(?)微球
It has been demonstrated that there is nucleotide homology between the sequences of HBV and interferon-alpha (IFN) -induced antiviral systems. In this paper, the authors investigated whether the presence or absence of different copies of the HBV genome in transfected cells reduces the sensitivity of transfected cells to IFN. The authors constructed plasmids that lacked the HBV genome and contained two, 12 copies of the HBV subtype ayw genome and used these plasmids to transfect IFN-sensitive human amniotic membrane cell lines, FL5-1 and HeLa cells. The inhibitory rate was calculated and the antiviral response was estimated by measuring the inhibitory effect of transfected FL5-1 cells on the pathogenicity of Sindbis virus before and after induction of IFN and the levels of anti-viral in HeLa cells were determined by radioimmunoassay β_ (?) microspheres