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海甘蓝种子在附加有０～１０ｍｇ／Ｌ６—ＢＡ＋０．１ｍｇ／ＬＮＡＡ的ＭＳ培养基中，当６—ＢＡ为２～５ｍｇ／Ｌ时，幼苗生长健壮．采用ＭＳ＋２～４ｍｇ／Ｌ６—ＢＡ＋０．１ｍｇ／ＬＮＡＡ培养基可从无菌苗茎尖诱导大量丛生芽，２～３周后，平均每个茎尖可诱导５．１～５．５个丛生芽，６—ＢＡ大于４ｍｇ／Ｌ，会导致丛芽黄化．继代培养时，４ｍｇ／Ｌ６ＢＡ＋０．１ｍｇ／ＬＮＡＡ配合使用效果较佳，繁殖系数高达１０．４．１／２ＭＳ＋０．５ｍｇ／ＬＩＢＡ丛芽的生根效果较好，保湿组培苗的成活率可达９５％，再生植株在自然条件下能正常开花结实．
In MS medium supplemented with 0 ~ 10 mg / L 6-BA + 0.1 mg / L NAA, seedlings grew robust when 6-BA was 2 ~ 5 mg / L. MS + 2-4mg / L 6-BA + 0.1mg / L NAA medium can induce a large number of shoots from the apex of aseptic shoots. After 2 ~ 3 weeks, 5.1 ~ 5.5 shoots per shoot were induced on average, 6-BA greater than 4mg / L, will lead to plexus yellowing. In the subculture, 4mg / L 6BA + 0.1mg / LNAA was better, with the multiplication coefficient up to 10.4.1 / 2MS + 0.5mg / LIBA bud rooting effect is better, the survival rate of moisturizing tissue culture seedlings can reach 95 %, Regenerated plants can flowering and fruiting under natural conditions.