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一、引言检验玉米杂交种种子批纯度的方法是将种子批样品种于大田条件下,确定母本的自交频率数值。一般情况下,大田生长试验中的自交植株其营养生长和生殖生长表现较差,因此可以辩别出被公认的自交株。最近已利用电泳测定幼苗的同功酶谱带型鉴定母本自交,因为自交幼苗中缺少父本编码的酶带。玉米醇溶胚乳蛋白(玉米朊)的等电聚集电泳也能分辨出自交系和杂交种,可用于评价种子批的纯度。
I. INTRODUCTION The method for testing the purity of seed batches of maize hybrids is to seed the seed batches under field conditions and determine the self-fertilization frequency of the maternal parent. In general, self-cross-fertilized plants in field-grown experiments showed poor performance in vegetative growth and reproductive growth, and thus it was possible to discern recognized self-crossings. Maize selfing has recently been identified by zymogram identification of seedlings by electrophoresis, as maternal-derived bands are absent in selfed seedlings. Isoelectric focusing of corn alcohol-soluble endosperm protein (zein) can also distinguish inbred lines and hybrids and can be used to evaluate the purity of seed batches.