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目的:通过模式动物研究溶酶体膜蛋白Sidt2与糖代谢的关系。方法:利用Lox P-Flox-Lox P系统,通过基因重组及打靶技术获得Sidt2剔除小鼠模型。检测成年小鼠的空腹血糖及葡萄糖耐量,评估Sidt2基因剔除对小鼠糖代谢的影响。同时通过HE染色观察Sidt2-/-小鼠胰岛形态结构改变。结果:通过对Sidt2剔除模型小鼠的Sidt2 mRNA及蛋白水平鉴定,确定Sidt2基因剔除小鼠模型成功构建。对小鼠末梢血糖检测发现,Sidt2-/-成年小鼠表现出空腹血糖增高及糖耐量受损的糖尿病表现。对Sidt2-/-小鼠胰岛形态检测发现,其胰岛出现结构紊乱,胰岛空泡变性及部分细胞出现肥大等病理改变。结论:溶酶体膜蛋白Sidt2与糖代谢密切相关。
OBJECTIVE: To study the relationship between lysosomal membrane protein Sidt2 and glucose metabolism in model animals. Methods: The Sox2 knockout mouse model was obtained by gene recombination and targeting using the Lox P-Flox-Lox P system. Fasting plasma glucose and glucose tolerance were measured in adult mice to assess the effect of Sidt2 knockout on glucose metabolism in mice. Meanwhile, the morphological changes of islets in Sidt2 - / - mice were observed by HE staining. Results: Sidt2 knockout mouse model of Sidt2 mRNA and protein levels identified to determine the Sidt2 knockout mouse model was successfully constructed. In mouse peripheral blood glucose test, Sidt2 - / - adult mice showed elevated fasting blood glucose and impaired glucose tolerance of diabetic performance. Sidt2 - / - mouse pancreatic islet morphology test found that its islet structure disorder, islet cell degeneration and some cells hypertrophy and other pathological changes. Conclusion: The lysosomal membrane protein Sidt2 is closely related to glucose metabolism.