论文部分内容阅读
光镊利用光学梯度力捕获和操控微小粒子,已经成为深入研究生物分子间相互作用等微观机制的独特技术.光镊光束操控系统一般由扩束输入镜、扩束输出镜、调焦透镜、耦合透镜和压电转镜等光学元器件组成,以保证物镜后瞳充满的前提下实现光镊阱位操控.光镊阱位的三维精确操控是实现光镊位钳和力钳模式的基本条件.本文根据矩阵光学,对基于无穷远校正显微镜的光镊操控光路进行计算,分析扩束输入镜、调焦透镜和物镜轴向位置调整,以及压电转镜、调焦透镜和耦合透镜安装位置误差对光镊径向阱位操控精度的影响,得到了物镜高度调整基本不会影响光镊径向位置操控,压电转镜和调焦透镜的安装位置误差对光镊径向阱位操控精度影响最大等结论,提出了能够实现径向阱位精确操控的轴向阱位动态操控范围,为光镊设计和操控提供理论和实验指导.
Optical tweezers using optical gradient force capture and control of small particles, has become a deep study of the interaction between biological molecules and other micro-mechanisms of unique technology.Optical tweezers beam manipulation system generally by the beam expander input mirror, beam expander lens, focusing lens, coupling Lens and piezoelectric mirror and other optical components in order to ensure that the lens under the premise of full pupil after the tweezers trapped manipulation .Three-dimensional precise control of optical tweezers well position is the basic conditions for the implementation of optical tweezers plier and forceps mode. According to the matrix optics, the optical path of the optical tweezers based on the infinity correction microscope is calculated, and the position adjustment of the beam expander input lens, the focus lens and the objective lens and the position error of the piezoelectric rotary lens, the focusing lens and the coupling lens are analyzed The influence of optical tweezers on the well position control precision is obtained, the height adjustment of the objective lens is basically not affected the position control of the optical tweezers, and the error of the installation position of the piezoelectric rotating lens and the focusing lens affects the accuracy of the optical well position control Maximum and other conclusions, put forward to achieve the precise control of the radial well position of the axial trap dynamic range of control, optical tweezers design and manipulation provide theoretical and experimental guidance.