论文部分内容阅读
目的:探讨RNA干扰技术对人卵巢癌细胞SKOV3中mTOR的表达及对细胞增殖与凋亡的影响。方法:培养SKOV3细胞系,设计合成mTOR siRNA实验分4组:正常培养组:未转染的正常培养SKOV3细胞;空白对照组:转染空脂质体的SKOV3细胞;转染组:转染mTOR siRNA的SKOV3细胞;无义对照组:转染无义siRNA的SKOV3细胞。采用Western blot法检测各组细胞中的mTOR蛋白表达水平;应用MTT法检测细胞增殖;流式细胞仪检测细胞凋亡。结果:mTOR siRNA转染组SK-OV3细胞mTOR蛋白的表达显著低于各对照组(P<0.05);mTOR siRNA转染组SKOV3细胞增殖低于各对照组(P<0.05);mTOR siRNA转染组细胞凋亡率高于各对照组(P<0.05)。结论:mTOR siRNA对SKOV3细胞中mTOR蛋白的表达有明显的抑制作用,特异性阻断mTOR基因表达可显著抑制SKOV3细胞的增殖并促进其凋亡。
Objective: To investigate the effect of RNAi on mTOR expression and cell proliferation and apoptosis in human ovarian cancer cell line SKOV3. Methods: The SKOV3 cell line was cultured and the mTOR siRNA was designed and synthesized into four groups: normal culture group: untransfected normal cultured SKOV3 cells; blank control group: SKOV3 cells transfected with empty liposomes; transfected group: transfected mTOR siRNA; SKOV3 cells; nonsense control group: SKOV3 cells transfected with nonsense siRNA. Western blot was used to detect the expression of mTOR protein in each group. Cell proliferation was detected by MTT assay. Apoptosis was detected by flow cytometry. Results: The mTOR protein expression in SK-OV3 cells transfected with mTOR siRNA was significantly lower than that in control cells (P <0.05). The proliferation of SKOV3 cells in mTOR siRNA group was lower than that in control cells (P <0.05) The rate of apoptosis in group was higher than that in control group (P <0.05). CONCLUSION: mTOR siRNA can significantly inhibit the expression of mTOR protein in SKOV3 cells. Blocking the expression of mTOR gene specifically inhibits the proliferation and induces the apoptosis of SKOV3 cells.