论文部分内容阅读
目的建立一种超高效液相色谱-三重四极杆/复合线性离子阱质谱(ultra performance liquid chromatography-triple quadrupole linear/ion trap of mass spectrometry,UPLC-Qtrap-MS/MS)检测方法,用于保健食品中西地那非类衍生物的快速筛查和鉴定。方法样品经甲醇提取,经甲醇-水(50:50,V:V)适当稀释,采用0.1%甲酸水(A)和0.1%甲酸乙腈(B)作为流动相进行洗脱,质谱(ESI+)采用母离子扫描模式(precursor ion)对西地那非类似物进行检测,利用一级、二级、三级质谱碎片进行结构解析。结果通过该方法成功发现了一种新型的西地那非类衍生物。结论该方法快速、准确、灵敏,可用于保健食品中西地那非类衍生物的筛查。
Objective To establish a ultra performance liquid chromatography-triple quadrupole linear / ion trap of mass spectrometry (UPLC-Qtrap-MS / MS) Rapid Screening and Identification of Sildenafil Derivatives in Foods. Methods The samples were extracted with methanol, diluted with methanol-water (50:50, V: V), eluted with 0.1% formic acid water and 0.1% formic acid as mobile phase, Precursor ion The sildenafil analogs were detected and structural elucidation was performed using first-, second-, and third-order MS fragments. Results A novel sildenafil derivative was successfully discovered by this method. Conclusion The method is rapid, accurate and sensitive and can be used for the screening of sildenafil derivatives in health foods.