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目的 探讨神经元型一氧化氮合酶 (neuronalnitricoxidesynthase,nNOS)及一氧化氮 (nitricoxide ,NO)在学习记忆机制中的相关作用。方法 采用免疫组化方法观察Y迷宫空间辨别学习训练后大鼠不同脑区nNOS表达变化 ,并探讨特异性nNOS抑制剂 7 nitroindozal(7 NI)、钙拮抗剂尼莫通 (nimotop)腹腔注射对大鼠学习获得和记忆再现能力的影响。结果 学习训练后海马各亚区nNOS样神经元数量及染色强度明显增加 ,而皮层和纹状体区则无显著变化 ;7 NI以剂量依赖方式损伤大鼠的学习获得能力 ,但不影响记忆再现 ,尼莫通则对这两种能力均有破坏。结论 提示学习记忆过程可能伴有nNOS合成及活性增加 ,nNOS/NO在学习获得阶段具有重要作用。
Objective To investigate the role of neuronal nitric oxide synthase (nNOS) and nitric oxide (NO) in learning and memory. Methods Immunohistochemistry was used to observe the changes of nNOS expression in different brain regions of rats after Y-maze spatial discrimination learning training. The effects of specific nNOS inhibitor 7-nitroindozal (7 NI) and calcium-antagonist nimotop Effect of Learning Acquisition and Memory Reproducibility in Rats. Results After training, the number and intensity of nNOS-like neurons in hippocampus significantly increased, while there was no significant change in cortex and striatum. 7 NI attenuated the learning ability of rats in a dose-dependent manner but did not affect the memory reproduction Nimotong is both destructive of these two capabilities. Conclusion suggests that learning and memory process may be associated with increased nNOS synthesis and activity, nNOS / NO plays an important role in the learning process.